ML734 pSP6-nsP1-nsP3 (with ALI mutations)-P2A-neoR-EGFP-P2A-

LOCUS       ML734 pSP6-nsP1-nsP3 (with ALI mutations)-P2A-neoR-EGFP-P2A-PuroR             10674 bp DNA     circular SYN 18-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ML734 ML708 3M(ALI)-neo-GFP-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10674)
  AUTHORS   maliang
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             60..1679
                     /codon_start=1
                     /label=nsP1
                     /note="nsP1"
                     /translation="MEKPVVNVDVDPQSPFVVQLQKSFPQFEVVAQQVTPNDHANARAF
                     SHLASKLIELEVPTTATILDIGSAPARRMFSEHQYHCVCPMRSPEDPDRMMKYASKLAE
                     KACKITNKNLHEKIKDLRTVLDTPDAETPSLCFHNDVTCNTRAEYSVMQDVYINAPGTI
                     YHQAMKGVRTLYWIGFDTTQFMFSAMAGSYPAYNTNWADEKVLEARNIGLCSTKLSEGR
                     TGKLSIMRKKELKPGSRVYFSVGSTLYPEHRASLQSWHLPSVFHLKGKQSYTCRCDTVV
                     SCEGYVVKKITISPGITGETVGYAVTNNSEGFLLCKVTDTVKGERVSFPVCTYIPATIC
                     DQMTGIMATDISPDDAQKLLVGLNQRIVINGKTNRNTNTMQNYLLPIIAQGFSKWAKER
                     KEDLDNEKMLGTRERKLTYGCLWAFRTKKVHSFYRPPGTQTIVKVPASFSAFPMSSVWT
                     TSLPMSLRQKIKLALQPKKEEKLLQVPEELVMEAKAAFEDAQEESRAEKLREALPPLVA
                     DKGIEAAAEVVCEVEGLQADIAA"
     misc_feature    1674..1676
                     /label=G539A
                     /note="G539A"
     CDS             1680..4100
                     /codon_start=1
                     /label=nsP2
                     /note="nsP2"
                     /translation="ALVETPRGHVRIIPQANDRMIGQYIVVSPTSVLKNAKLAPAHPLA
                     DQVKIITHSGRSGRYAVEPYDAKVLMPAGSAVPWPEFLALSESATLVYNEREFVNRKLY
                     HIAMHGPAKNTEEEQYKVTKAELAETEYVFDVDKKRCVKKEEASGLVLSGELTNPPYHE
                     LALEGLKTRPAVPYKVETIGVIGTPGSGKSAIIKSTVTARDLVTSGKKENCREIEADVL
                     RLRGMQITSKTVDSVMLNGCHKAVEVLYVDEAFACHAGALLALIAIVRPRKKVVLCGDP
                     KQCGFFNMMQLKVYFNHPEKDICTKTFYKFISRRCTQPVTAIVSTLHYDGKMKTTNPCK
                     KNIEIDITGATKPKPGDIILTCFRGWVKQLQIDYPGHEVMTAAASQGLTRKGVYAVRQK
                     VNENPLYAITSEHVNVLLTRTEDRLVWKTLQGDPWIKQLTNVPKGNFQATIEDWEAEHK
                     GIIAAINSPAPRTNPFSCKTNVCWAKALEPILATAGIVLTGCQWSELFPQFADDKPHSA
                     IYALDVICIKFFGMDLTSGLFSKQSIPLTYHPADSARPVAHWDNSPGTRKYGYDHAVAA
                     ELSRRFPVFQLAGKGTQLDLQTGRTRVISAQHNLVPVNRNLPHALVPEHKEKQPGPVKK
                     FLSQFKHHSVLVVSEEKIEAPHKRIEWIAPIGIAGADKNYNLAFGFPPQARYDLVFINI
                     GTKYRNHHFQQCEDHAATLKTLSRSALNCLNLGGTLVVKSYGYADRNSEDVVTALARKF
                     VRVSAARPECVSSNTEMYLIFRQLDNSRTRQFTPHHLNCVISSVYEGTRDGVIA"
     exon            3855..3857
                     /note="P726L"
     misc_feature    4095..4097
                     /label=G806I
                     /note="G806I"
     CDS             4101..5762
                     /codon_start=1
                     /label=nsP3
                     /note="nsP3"
                     /translation="APSYRTKRENIADCQEEAVVNAANPLGRPGEGVCRAIYKRWPNSF
                     TDSATETGTAKLTVCQGKKVIHAVGPDFRKHPEAEALKLLQNAYHAVADLVNEHNIKSV
                     AIPLLSTGIYAAGKDRLEVSLNCLTTALDRTDADVTIYCLDKKWKERIDAVLQLKESVT
                     ELKDEDMEIDDELVWIHPDSCLKGRKGFSTTKGKLYSYFEGTKFHQAAKDMAEIKVLFP
                     NDQESNEQLCAYILGETMEAIREKCPVDHNPSSSPPKTLPCLCMYAMTPERVHRLRSNN
                     VKEVTVCSSTPLPKYKIKNVQKVQCTKVVLFNPHTPAFVPARKYIEAPEQPAAPPAQAE
                     EAPEVAATPTPPAADNTSLDVTDISLDMEDSSEGSLFSSFSGSDNSITSMDSWSSGPSS
                     LEIVDRRQVVVADVHAVQEPAPVPPPRLKKMARLAAARMQEEPTPPASTSSADESLHLS
                     FGGVSMSFGSLFDGEMARLAAAQPPASTCPTDVPMSFGSFSDGEIEELSRRVTESEPVL
                     FGSFEPGEVNSIISSRSAVSFPPRKQRRRRRSRRTEY"
     CDS             5763..5819
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1
                     polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5820..6614
                     /codon_start=1
                     /label=neo
                     /note="neoR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      6947..6956
                     /label=Kozak sequence
                     /note="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6953..7669
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=the original enhanced GFP (Yang et al., 1996)
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             7670..7726
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1
                     polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7733..8329
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=pac from Streptomyces alboniger
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     gene            8330..8332
                     /gene="UL48"
                     /label=UL48
                     /note="UL48"
     misc_feature    8695..8778
                     /label=antigenomic ribozyme HDV
                     /note="antigenomic ribozyme HDV"
     promoter        8845..8949
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             8950..9810
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9981..10569
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        join(10657..10674,1)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 attggcggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagttaac gtagacgtag acccgcagag tccgtttgtc gtgcaactgc
      121 aaaagagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatttt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtacc
      301 attgcgtttg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagca
      361 aactggcgga aaaagcatgc aagattacga ataagaactt gcatgagaag atcaaggacc
      421 tccggaccgt acttgataca ccggatgctg aaacgccatc actctgcttc cacaacgatg
      481 ttacctgcaa cacgcgtgcc gagtactccg tcatgcagga cgtgtacatc aacgcacccg
      541 gaactattta ccatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgata
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgaaaa agtcctcgaa gcgcgtaaca tcggactctg cagcacaaag ctgagtgaag
      721 gcaggacagg aaagttgtcg ataatgagga agaaggagtt gaagcccggg tcacgggttt
      781 atttctccgt tggatcgaca ctttacccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacctg aaaggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagctgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca aacaatagcg agggcttctt gctatgcaaa gttaccgata
     1021 cagtaaaagg agaacgggta tcgttccccg tgtgcacgta tatcccggcc accatatgcg
     1081 atcagatgac cggcataatg gccacggata tctcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatc gtcattaacg gtaagactaa caggaacacc aataccatgc
     1201 aaaattacct tctgccaatc attgcacaag ggttcagcaa atgggccaag gagcgcaaag
     1261 aagaccttga caatgaaaaa atgctgggta ccagagagcg caagcttaca tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtgcact cgttctatcg cccacctgga acgcagacca
     1381 tcgtaaaagt cccagcctct tttagcgctt tccccatgtc atccgtatgg actacctctt
     1441 tgcccatgtc gctgaggcag aagataaaat tggcattaca accaaagaag gaggaaaaac
     1501 tgctgcaagt cccggaggaa ttagtcatgg aggccaaggc tgctttcgag gatgctcagg
     1561 aggaatccag agcggagaag ctccgagaag cactcccacc attagtggca gacaaaggta
     1621 tcgaggcagc cgcggaagtt gtctgcgaag tggaggggct ccaggcggac atcgctgcag
     1681 cactcgtcga aaccccgcgc ggtcatgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta catcgttgtc tcgccaacct ctgtgctgaa gaacgctaaa ctcgcaccag
     1801 cacacccgct agcagaccag gttaagatca taacgcactc cggaagatca ggaaggtatg
     1861 cagtcgaacc atacgacgct aaagtactga tgccagcagg aagtgccgta ccatggccag
     1921 aattcttagc actgagtgag agcgccacgc tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaagctgta ccatattgcc atgcacggtc ccgctaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctc gcagaaacag agtacgtgtt tgacgtggac aagaagcgat
     2101 gcgtcaagaa ggaagaagcc tcaggacttg tcctctcggg agaactgacc aacccgccct
     2161 atcacgaact agctcttgag ggactgaaga ctcgacccgc ggtcccgtac aaggttgaaa
     2221 caataggagt gataggcaca ccaggatcgg gcaagtcggc tatcatcaag tcaactgtca
     2281 cggcacgtga tcttgttacc agcggaaaga aagaaaactg ccgcgaaatt gaggccgatg
     2341 tgctacggct gaggggcatg cagatcacgt cgaagacagt ggattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtatg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgca atcgtcagac cccgtaagaa ggtagtgcta tgcggagacc
     2521 ctaagcaatg cggattcttc aacatgatgc aactaaaggt atatttcaac cacccggaaa
     2581 aagacatatg taccaagaca ttctacaagt ttatctcccg acgttgcaca cagccagtca
     2641 cggctattgt atcgacactg cattacgatg gaaaaatgaa aaccacaaac ccgtgcaaga
     2701 agaacatcga aatcgacatt acaggggcca cgaagccgaa gccaggggac atcatcctga
     2761 catgcttccg cgggtgggtt aagcaactgc aaatcgacta tcccggacat gaggtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtata tgccgtccgg caaaaagtca
     2881 atgaaaaccc gctgtacgcg atcacatcag agcatgtgaa cgtgctgctc acccgcactg
     2941 aggacaggct agtatggaaa actttacagg gcgacccatg gattaagcag ctcactaacg
     3001 taccaaaagg aaattttcaa gccaccatcg aggactggga agctgaacac aagggaataa
     3061 ttgctgcgat aaacagtccc gctccccgta ccaatccgtt cagctgcaag actaacgttt
     3121 gctgggcgaa agcactggaa ccgatactgg ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga gctgttccca cagtttgcag atgacaaacc acactcggcc atctacgccc
     3241 tggacgtaat ctgcattaag tttttcggca tggacttgac aagcggactg ttttccaaac
     3301 agagcatccc gttaacgtac catcctgccg attcagcgag gccagtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccgt tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaaag gcacacagct tgatttgcag acgggcagaa
     3481 ctagagttat ctccgcacag cataacttgg tcccagtgaa ccgcaatctc ccgcacgcct
     3541 tagtccccga gcacaaggag aaacaacccg gcccggtcaa aaaattcttg agccagttca
     3601 aacaccactc cgtacttgtg gtctcagagg aaaaaattga agctccccac aagagaatcg
     3661 aatggatcgc cccgattggc atagccggcg ctgataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt ttatcaatat tggaactaaa tacagaaacc
     3781 atcactttca gcagtgcgaa gaccatgcgg cgaccttgaa aaccctctcg cgttcggccc
     3841 tgaactgcct taaccttgga ggcaccctcg tggtgaagtc ctacggttac gccgaccgca
     3901 atagtgagga cgtagtcacc gctcttgcca gaaaatttgt cagagtgtct gcagcgaggc
     3961 cagagtgcgt ctcaagcaat acagaaatgt acctgatctt ccgacaacta gacaacagcc
     4021 gcacacgaca attcaccccg catcatctga attgtgtgat ttcgtccgtg tacgagggta
     4081 caagagacgg agttattgcc gcaccgtcat accgcactaa aagggagaac attgctgatt
     4141 gtcaagagga agcagttgtc aatgcagcca atccgctggg cagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgaaca gtttcaccga ttcagccaca gagaccggca
     4261 ccgcaaaact gactgtgtgc caaggaaaga aagtgatcca cgcggttggc cctgatttcc
     4321 ggaaacaccc agaggcagaa gccctgaaat tgctgcaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataat atcaagtctg tcgccatccc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagata
     4501 gaactgatgc ggacgtaacc atctactgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggtgctcca acttaaggag tctgtaacag agctgaagga tgaggatatg gagatcgacg
     4621 acgagttagt atggatccat ccggacagtt gcctgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa gttgtattcg tactttgaag gcaccaaatt ccatcaagca gcaaaagata
     4741 tggcggagat aaaggtcctg ttcccaaatg accaggaaag caacgagcaa ctgtgtgcct
     4801 acatattggg ggagaccatg gaagcaatcc gcgaaaaatg cccggtcgac cacaacccgt
     4861 cgtctagccc gccaaaaacg ctgccgtgcc tctgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact cagaagcaac aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 caaagtacaa aatcaagaac gttcagaagg ttcagtgcac aaaagtagtc ctgtttaacc
     5041 cgcatacccc tgcattcgtt cccgcccgta agtacataga agcgccagaa cagcctgcag
     5101 ctccgcctgc acaggccgag gaggcccccg aagttgcagc aacaccaaca ccacctgcag
     5161 ctgataacac ctcgcttgat gtcacggaca tctcactgga catggaagac agtagcgaag
     5221 gctcactctt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgtcc
     5341 atgccgtcca agagcctgcc cctgttccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aatgcaggaa gagccaactc caccggcaag caccagctct gcggacgagt
     5461 cccttcacct ttcttttggt ggggtatcca tgtccttcgg atcccttttc gacggagaga
     5521 tggcccgctt ggcagcggca caacccccgg caagtacatg ccctacggat gtgcctatgt
     5581 ctttcggatc gttttccgac ggagagattg aggagctgag ccgcagagta accgagtctg
     5641 agcccgtcct gtttgggtca tttgaaccgg gcgaagtgaa ctcaattata tcgtcccgat
     5701 cagccgtatc ttttccacca cgcaagcaga gacgtagacg caggagcagg aggaccgaat
     5761 acgcaacaaa cttctcacta ctcaaacaag caggtgacgt ggaggagaat cccgggccta
     5821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     5881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     5941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     6001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     6061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     6121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     6181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     6241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     6301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     6361 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     6421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     6481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     6541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     6601 acgagttctt ctgatagggt accaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     6661 acgatgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     6721 gagtaggtat aacagacacc ttagcagtgg ccgtggcaac tcggtatgag gtagacaaca
     6781 tcacacctgt cctgctggca ttgagaactt ttgcccagag caaaagagca tttcaagcca
     6841 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tacatttcat
     6901 ctgactaata ccacaacacc accagcggcc gcatcgatca ccggtcgcca ccatggtgag
     6961 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     7021 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     7081 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     7141 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     7201 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     7261 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     7321 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     7381 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     7441 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     7501 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     7561 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     7621 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg caacaaactt
     7681 ctcactactc aaacaagcag gtgacgtgga ggagaatccc gggccttcta gaatgaccga
     7741 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     7801 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     7861 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     7921 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     7981 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8041 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     8101 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     8161 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     8221 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     8281 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agcctaggag
     8341 cggcatcgat actagtacgc cccaatgacc cgaccagcaa aactcgatgt acttccgagg
     8401 aactgatgtg cataatgcat caggctggta tattagatcc ccgcttaccg cgggcaatat
     8461 agcaacacca aaactcgacg tatttccgag gaggcgcagt gcataatgct gcgcagtgtt
     8521 gccaaataat cactatatta accatttatt cagcggacgc caaaactcaa tgtatttctg
     8581 aggaagcatg gtgcataatg ccatgcagcg tctgcataac tttttattat ttcttttatt
     8641 aatcaacaaa attttgtttt taacatttca aaaaaaaaaa aaaaaaaaaa aaaagggtcg
     8701 gcatggcatc tccacctcct cgcggtccga cctgggcatc cgaaggagga cgcacgtcca
     8761 ctcggatggc taagggaggg ggatctcgag gggaattaat tcttgaagac gaaagggcca
     8821 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8881 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8941 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     9001 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     9061 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     9121 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     9181 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     9241 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     9301 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     9361 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     9421 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9481 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9541 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9601 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9661 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9721 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9781 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9841 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9901 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9961 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    10021 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    10081 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
    10141 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    10201 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    10261 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    10321 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    10381 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    10441 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10501 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10561 ctatggaaaa acgccagcaa cgcgagctcg tatggacata ttgtcgttag aacgcggcta
    10621 caattaatac ataaccttat gtatcataca catacgattt aggtgacact atag
//